Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Hela, HepG2 and A2780 cells using HSF1 Antibody .Application
Western blot analysis of extracts from Hela, HepG2 and A2780 cells using HSF1(Ab-307) Antibody| Product Name | HSF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSF1DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. |
| Immunogen | Peptide sequence around aa. 305~309 (P-Q-S-P-R) derived from Human HSF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HSTF1 HSF1 HSTF 1 heat shock factor protein 1 HSF 1 heat shock transcription factor 1 |
|---|---|
| Target | HSF1 |
| Molecular Weight(MW) | 82 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Hela, HepG2 and A2780 cells using HSF1 Antibody .
Application
Western blot analysis of extracts from Hela, HepG2 and A2780 cells using HSF1(Ab-307) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
