HSH2D antibody detects HSH2D protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HSH2D antibody diluted by 1:500.
Rabbit Polyclonal antibody to HSH2D (hematopoietic SH2 domain containing)T-cell activation requires 2 signals: recognition of antigen by the T-cell receptor (see TCR; MIM 186880) and a costimulatory signal provided primarily by CD28 (MIM 186760) in naive T cells. HSH2 is a target of both of these signaling pathways (Greene et al., 2003 [PubMed 12960172]).[supplied by OMIM]
Immunogen
Recombinant protein encompassing a sequence within the center region of human HSH2D. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
:
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
HSH2 ALX hematopoietic SH2 domain containing
Target
HSH2D
Molecular Weight(MW)
39 kDa (note) (2)
Cellular Localization
Cytoplasm , Nucleus
Application
Application
HSH2D antibody detects HSH2D protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with HSH2D antibody diluted by 1:500.
Positive Control
NCI-H929
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.