Application
Flow cytometric analysis of NCI-H292 cells using ALKBH8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human spleen reacted with ALKBH8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of ALKBH8 Antibody (C-term) in HL60 cell line lysates (35ug/lane). ALKBH8 (arrow) was detected using the purified Pab.| Product Name | Hsp 60 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Hsp 60 (N-term) |
| Immunogen | KLH conjugated synthetic peptide between 87~117 amino acids from the N-terminal region of human Hsp60. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| FACS:1:50~1:100 IHC-P:1:10~1:50 WB:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Heat shock 70 kDa protein 14 HSP60 HSPA14 Heat shock70 kDa protein14 HSP70like protein1 HSP70-like protein 1 HSP70like protein 1 HSP70L1 Heat shock protein HSP60 HSP70 like protein 1 |
|---|---|
| Target | Hsp 60 |
| Molecular Weight(MW) | 54.794 kDa (note) |
Application
Flow cytometric analysis of NCI-H292 cells using ALKBH8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human spleen reacted with ALKBH8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of ALKBH8 Antibody (C-term) in HL60 cell line lysates (35ug/lane). ALKBH8 (arrow) was detected using the purified Pab.| Application Notes | FACS:1:50~1:100 IHC-P:1:10~1:50 WB:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
