WB
Western blot analysis using HSP60 antibody against A549(1), Mcf-7(2), K562(3), A431(4), Cos-7(5), Hepg2(6), Hela(7) cell lysate.12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-HSP60 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HSP60 |
|---|---|
| Gene Synonyms | 60kDa CPN60 GROEL HLD4 HSP-60 HSP-65 HSP60 HSP65 Hspd1-30p HuCHA60 I79_011398 MIF4 MNA2 SPG13 YLR259C |
| Gene Full Name | heat shock protein family D (Hsp60) member 1 |
| Gene Summary | This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010] |
| Alternative Names | 60 kDa chaperonin 60 kDa heat shock protein a stress-inducible molecular chaperone which participates in protein folding chaperonin chaperonin (HSP60) Chaperonin 60 CPN60 epididymis secretory sperm binding protein heat shock 60 kDa protein 1 heat shock 60kD protein 1 (chaperonin) heat shock 60kDa protein 1 heat shock 60kDa protein 1 (chaperonin) heat shock 60kDa protein 1 (chaperonin) isoform HSPD1_1 heat shock 60kDa protein 1 (chaperonin) isoform HSPD1_2 heat shock 60kDa protein 1 (chaperonin) isoform HSPD1_4 Heat shock protein heat shock protein 1 (chaperonin) Heat shock protein 60 heat shock protein 60 (liver) heat shock protein 65 heat shock protein family D member 1 heat shock protein 60 kDa HSP HSP-60 HSP-65 hsp60 Hsp60s1 mitochondrial heat shock 60 kDa protein 1 Mitochondrial matrix protein P1 P1 protein P60 lymphocyte protein short heat shock protein 60 Hsp60s1 |
| Molecular Weight(MW) | 61KD |
| Cellular Localization | Mitochondrion |
| SwissProt ID | P10809 |
|---|
WB
Western blot analysis using HSP60 antibody against A549(1), Mcf-7(2), K562(3), A431(4), Cos-7(5), Hepg2(6), Hela(7) cell lysate.12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
