| Product Name | Hsp25 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [HSP25-26] to Hsp25In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. One class of stress proteins, termed the "small heat shock proteins" is comprised of a diverse group of proteins from ~15 to >30kDa, with a single form found in yeast and human cells and multiple related forms found in higher plants. A direct relationship between elevated stress protein expression and neoplasia has been suggested from studies on estrogen-induced proteins in breast cancer cells. In addition, Hsp27 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores. The expression of a 24kDa protein was shown to correlate with steroid hormone receptors in cell lines, organs , and tumors. The 24kDa estrogen-induced protein was found to be expressed at high levels in carcinoma of the breast. Another study has suggested that the levels of Hsp27 correlate with endometrial adenocarcinomas. Interestingly, the regulation of human Hsp27 by estrogens and heat shock is reminiscent of the ecdysteroid and heat shock activation of the drosophila Hsp20 genes. |
| Immunogen | Recombinant HSP25 (Chicken). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | HSP25-26 |
| Host Species | Mouse |
| Tested Applications | PurifyELISAICC/IFIPWB |
| HSP-25/27 AP: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. IF: Staining of cell cultures. Use at an assay dependent dilution. IP: HSP-25/27 from cell/tissue extracts. Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 27 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMouseDogPigRatCatFishGoatGuinea Pig |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | Heat Shock Protein25 Hsp25 HSPB1 Heat Shock Protein 25 |
|---|---|
| Target | Hsp25 |
| Tissue Specificity | Cross reacts with HSP25/27 from mouse, guinea pig, rat, dog, cat, goat, chicken, fish, porcine and human. The amino acid sequence homology between HSP-25 (mouse) and HSP-27 (human) is >80%. |
| Cellular Localization | Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock. |
| Application Notes | HSP-25/27 AP: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. IF: Staining of cell cultures. Use at an assay dependent dilution. IP: HSP-25/27 from cell/tissue extracts. Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 27 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: 0.1% Sodium Azide. Constituents: PBS. pH 7.4 |
