Application
Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and cardiac muscle tissues (right) using HSP27 mouse mAb with DAB stainingApplication
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)Application
Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jurkat (3), A431 (4), HEK293(5), HepG2 (6) and PC-12 (7) cell lysateApplication
Immunohistochemical analysis of paraffin-embedded brain tissues (left) and esophageal cancer tissues (right) using HSP27 mouse mAb with DAB stainingApplication
Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Application
Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and negative control (purple).| Product Name | HSP27 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [5D7] to HSP27The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008] |
| Immunogen | The immunogen corresponding to human HSP27 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5D7 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Gene Synonyms | HMN2B HSP25 CMT2F HSPB1 HS.76067 SRP27 HSP28 |
|---|---|
| Target | HSP27 |
| Molecular Weight(MW) | 27 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and cardiac muscle tissues (right) using HSP27 mouse mAb with DAB staining
Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)
Application
Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jurkat (3), A431 (4), HEK293(5), HepG2 (6) and PC-12 (7) cell lysate
Application
Immunohistochemical analysis of paraffin-embedded brain tissues (left) and esophageal cancer tissues (right) using HSP27 mouse mAb with DAB staining
Application
Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Application
Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and negative control (purple).| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
