Application
HSP47 antibody [N2C2], Internal detects HSP47 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HSP47 antibody [N2C2], Internal dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HSP47 antibody [N2C2], Internal detects HSP47 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. HSP47 antibody [N2C2], Internal dilution: 1:500.Application
HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis. A. 30 µg A431 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 10% SDS-PAGE HSP47 antibody [N2C2], Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis. A. 30 µg U87-MG whole cell lysate/extract B. 30 µg SK-N-SH whole cell lysate/extract C. 30 µg IMR32 whole cell lysate/extract D. 30 µg SK-N-AS whole cell lysate/extract 10% SDS-PAGE HSP47 antibody [N2C2], Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | HSP47 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSP47 (serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1))This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSP47. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Chicken, Sheep, Rhesus Monkey, Chimpanzee, BovineRat (97%), Dog (98%), Pig (96%), Chicken (88%), Sheep (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AsTP3 antibody CBP1 antibody CBP2 antibody HSP47 antibody PPROM antibody RA-A47 antibody SERPINH2 antibody gp46 antibody SERPINH1 antibody rheumatoid arthritis antigen A-47 antibody colligin-1 antibody rheumatoid arthritis-related antigen RA-A47 |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum lumen |
Application
HSP47 antibody [N2C2], Internal detects HSP47 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HSP47 antibody [N2C2], Internal dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HSP47 antibody [N2C2], Internal detects HSP47 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. HSP47 antibody [N2C2], Internal dilution: 1:500.
Application
HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis. A. 30 µg A431 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg HepG2 whole cell lysate/extract 10% SDS-PAGE HSP47 antibody [N2C2], Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis. A. 30 µg U87-MG whole cell lysate/extract B. 30 µg SK-N-SH whole cell lysate/extract C. 30 µg IMR32 whole cell lysate/extract D. 30 µg SK-N-AS whole cell lysate/extract 10% SDS-PAGE HSP47 antibody [N2C2], Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | NIH-3T3 , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
