Application
HSP47 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
HSP47 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSP47 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of HSP47 Antibody (Center) in HepG2 cell line lysates (35ug/lane). HSP47 (arrow) was detected using the purified Pab.| Product Name | HSP47 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to HSP47 (Internal)This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 174~204 amino acids from the Center region of human HSP47. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Rheumatoid arthritisrelated antigen RAA47 Rheumatoid arthritis-related antigen RA-A47 Arsenic-transactivated protein 3 Cell proliferation-inducing gene 14 protein SER 47 kDa heat shock protein Arsenictransactivated protein3 Cell proliferationinducin |
|---|---|
| Target | SERPINH1 |
| Molecular Weight(MW) | 46.441 kDa (note) |
Application
HSP47 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
HSP47 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSP47 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of HSP47 Antibody (Center) in HepG2 cell line lysates (35ug/lane). HSP47 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
