Application
HSP60 antibody detects HSP60 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP60 protein stained by HSP60 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.Application
HSP60 antibody detects HSP60 protein at mitochondrion on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HSP60 antibody dilution: 1:500.Application
HSP60 antibody detects HSP60 protein at mitochondrion on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HSP60 antibody dilution: 1:500.Application
HSP60 antibody detects HSP60 protein at mitochondria on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HSP60 antibody dilution: 1:500.Application
HSP60 antibody detects HSP60 protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. HSP60 antibody dilution: 1:500.Application
HSP60 antibody detects HSPD1 protein by western blot analysis. A. 50 µg rat brain lysate/extract 7.5% SDS-PAGE HSP60 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed A431, using HSP60 (chaperonin) antibody at 1:200 dilution.Application
Sample (20 µg of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:20000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP60 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | HSP60 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSP60 (heat shock 60kDa protein 1 (chaperonin))This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSP60. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRatHamster |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineZebrafish (85%), Xenopus laevis (91%), Pig (99%), Chicken (94%), Rhesus Monkey (97%), Chimpanzee (99%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CPN60 antibody GROEL antibody HLD4 antibody HSP-60 antibody HSP60 antibody HSP65 antibody HuCHA60 antibody SPG13 antibody HSPD1 antibody 60 kDa chaperonin antibody mitochondrial matrix protein P1 antibody chaperonin 60 antibody short heat shock |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) (2) |
| Cellular Localization | Mitochondrion matrix |
Application
HSP60 antibody detects HSP60 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP60 protein stained by HSP60 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
Application
HSP60 antibody detects HSP60 protein at mitochondrion on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HSP60 antibody dilution: 1:500.
Application
HSP60 antibody detects HSP60 protein at mitochondrion on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HSP60 antibody dilution: 1:500.
Application
HSP60 antibody detects HSP60 protein at mitochondria on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HSP60 antibody dilution: 1:500.
Application
HSP60 antibody detects HSP60 protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. HSP60 antibody dilution: 1:500.
Application
HSP60 antibody detects HSPD1 protein by western blot analysis. A. 50 µg rat brain lysate/extract 7.5% SDS-PAGE HSP60 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed A431, using HSP60 (chaperonin) antibody at 1:200 dilution.
Application
Sample (20 µg of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:20000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP60 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | H1299 , HeLaS3 , Molt-4 , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
