Mouse monoclonal antibody [4E7] to HSP70This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]
Immunogen
Recombinant human HSP70 (amino acids 1-641) purified from Escherichia coli .
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4E7
Host Species
Mouse
Tested Applications
ELISAIHC-PWB
Western Blotting: 1/500 - 1/2000.:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
heat shock 70kDa protein 1B HSP70-2 HSPA1B HSP70-1B
Target
HSP70
Tissue Specificity
This antibody specifically recognises human heat shock protein 70 (HSP70).
Application
Application
IHC-P analysis of human eccrine gland tissue using HSP70 antibody [4E7].
Application
WB analysis of HeLa cell lysate using HSP70 antibody [4E7].
Application Notes
Western Blotting: 1/500 - 1/2000.:
Additional Information
Form
Liquid
Storage Instructions
Store at +4°C or at -20°C if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.