Application
HSP70 1A antibody immunoprecipitates HSP70 1A protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HSP70 1A protein by 2.5 μg of HSP70 1A antibody 12% SDS-PAGE The immunoprecipitated HSP70 1A protein was detected by HSP70 1A antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug D: 293T 1ug 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: 293T B: NIH-3T3 C: Mouse brain D: PC-12 E: Rat brain 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HSP70 1A antibody detects HSP70 1A protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HSP70 1A antibody diluted at 1:500.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using HSP70 1A antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
HSP70 1A antibody detects HSPA1A protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. HSP70 1A antibody dilution: 1:500.| Product Name | HSPA1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSP70 1A (heat shock 70kDa protein 1A)This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSP70 1A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Sheep, BovineXenopus laevis (85%), Sheep (99%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ54303 antibody FLJ54370 antibody FLJ54392 antibody FLJ54408 antibody FLJ75127 antibody HSP70-1 antibody HSP70-1A antibody HSP70I antibody HSP72 antibody HSPA1 antibody HSPA1B antibody HSPA1A antibody heat shock 70kD protein 1A antibody dnaK |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) |
Application
HSP70 1A antibody immunoprecipitates HSP70 1A protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HSP70 1A protein by 2.5 μg of HSP70 1A antibody 12% SDS-PAGE The immunoprecipitated HSP70 1A protein was detected by HSP70 1A antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug D: 293T 1ug 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: 293T B: NIH-3T3 C: Mouse brain D: PC-12 E: Rat brain 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HSP70 1A antibody detects HSP70 1A protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HSP70 1A antibody diluted at 1:500.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using HSP70 1A antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
HSP70 1A antibody detects HSPA1A protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. HSP70 1A antibody dilution: 1:500.| Positive Control | 293T , NIH-3T3 , Mouse brain , PC-12 , Rat brain , Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , *Mesenchymal stem cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
