Application
Flow cytometric analysis of Hela cells, using anti-HSPA1A antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA1A monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.Application
Flow cytometric analysis of Jurkat cells, using anti-HSPA1A antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Anti-HSPA1A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HSPA1AApplication
Immunofluorescent staining of HepG2 cells using anti-HSPA1A mouse monoclonal antibody .| Product Name | HSPA1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5F3] to HSPA1AIn cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. |
| Immunogen | Full length human recombinant protein of human HSPA1A (NP_005336) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5F3 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | HumanDogMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HSP72 HSPA1 HSP70-1 HSPA1B HSP701 HSPA1A |
|---|---|
| Target | HSPA1A |
| Tissue Specificity | is specific for human HSPA1A (NP_005336). |
| Cellular Localization | Localized in cytoplasmic mRNP granules containing untranslated mRNAs. |
Application
Flow cytometric analysis of Hela cells, using anti-HSPA1A antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA1A monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.
Application
Flow cytometric analysis of Jurkat cells, using anti-HSPA1A antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Anti-HSPA1A mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with HSPA1A
Application
Immunofluorescent staining of HepG2 cells using anti-HSPA1A mouse monoclonal antibody .| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
