Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA1B antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HSPA1B antibody detects HSPA1B protein at cytoplasm by immunofluorescent analysis. Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSPA1B protein stained by HSPA1B antibody diluted at 1:400. Blue: Hoechst 33342 staining.Application
HSP70 1B antibody detects HSP70 1B protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HSP70 1B antibody diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded human glioma, using HSP70 1B antibody at 1:100 dilution.| Product Name | HSPA1B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSP70 1B (heat shock 70kDa protein 1B)This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSP70 1B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevisMouse (96%), Xenopus laevis (84%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ54328 antibody HSP70-1B antibody HSP70-2 antibody HSPA1A antibody HSPA1B antibody heat shock 70kD protein 1B antibody heat shock 70 kDa protein 1/2 antibody HSP70.1/HSP70.2 antibody heat shock 70 kDa protein 1A/1B antibody HSP70-1/HSP70-2 antib |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA1B antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HSPA1B antibody detects HSPA1B protein at cytoplasm by immunofluorescent analysis. Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSPA1B protein stained by HSPA1B antibody diluted at 1:400. Blue: Hoechst 33342 staining.
Application
HSP70 1B antibody detects HSP70 1B protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HSP70 1B antibody diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded human glioma, using HSP70 1B antibody at 1:100 dilution.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
