Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody diluted at 1:10000.Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody diluted at 1:10000.Application
HSPA2 antibody detects HSPA2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HSPA2 antibody diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 7.5% SDS PAGE diluted at 1:10000Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 7.5% SDS PAGE diluted at 1:10000| Product Name | HSPA2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSPA2 (heat shock-related 70 kDa protein 2)In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSPA2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Chicken, Chimpanzee, BovineXenopus laevis (92%), Chicken (96%), Chimpanzee (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HSP70-2 antibody HSP70-3 antibody HSPA2 antibody heat shock-related 70 kDa protein 2 antibody heat shock 70kD protein 2 antibody heat shock 70kDa protein 2 antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody diluted at 1:10000.
Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA2 antibody diluted at 1:10000.
Application
HSPA2 antibody detects HSPA2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HSPA2 antibody diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 7.5% SDS PAGE diluted at 1:10000
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 7.5% SDS PAGE diluted at 1:10000| Positive Control | A549 , H1299 , HCT116 , MCF-7 , NIH-3T3 , BCL-1 , U87-MG , SK-N-SH , IMR32 , SK-N-AS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
