Application
Immunofluorescence analysis of NIH/3T3 cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dyeApplication
Immunohistochemical analysis of paraffin-embedded human breast cancer using HSP70 mAb with DAB staining.Application
Western blot analysis using HSP70 mAb against Hela (1) cell lysate.| Product Name | HSPA4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5A6] to HSP70 |
| Immunogen | The immunogen corresponding to a region within amino acids 422 and 609 of human HSP70. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5A6 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-PWB |
| Recommended Starting Dilutions: For ELISA: Use at a dilution of 1:10000. For WB: Use at a dilution of 1:500 - 1:2000. For IHC-P: Use at a dilution of 1:200 - 1:1000. For ICC/IF: Use at a dilution of 1:200 - 1:1000. Not yet tested in other applications. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HSPH2 HSP70RY HSPA4 HS24/P52 APG-2 RY |
|---|---|
| Target | HSP70 |
| Molecular Weight(MW) | 70 kDa (note) |
Application
Immunofluorescence analysis of NIH/3T3 cells using HSP70 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer using HSP70 mAb with DAB staining.
Application
Western blot analysis using HSP70 mAb against Hela (1) cell lysate.| Application Notes | Recommended Starting Dilutions: For ELISA: Use at a dilution of 1:10000. For WB: Use at a dilution of 1:500 - 1:2000. For IHC-P: Use at a dilution of 1:200 - 1:1000. For ICC/IF: Use at a dilution of 1:200 - 1:1000. Not yet tested in other applications. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
