Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using HSPA6 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using HSPA6 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:10000| Product Name | HSPA6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSPA6 (heat shock 70kDa protein 6 (HSP70B'))In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSPA6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HSPA6 antibody heat shock 70 kDa protein 6 antibody heat shock 70 kDa protein B' antibody heat shock 70kD protein 6 (HSP70B') antibody heat shock 70kDa protein 6 (HSP70B') antibody |
|---|---|
| Molecular Weight(MW) | 71 kDa (note) (2) |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using HSPA6 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using HSPA6 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:10000| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
