Application
HSPC142 antibody detects C19orf62 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human C19orf62-transfected 293T cells 10 % SDS-PAGE HSPC142 antibody dilution: 1:10000| Product Name | HSPC142 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSPC142 (BRISC and BRCA1 A complex member 1)Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human HSPC142. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rhesus Monkey, BovineMouse (80%), Rhesus Monkey (98%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C19orf62 antibody FLJ20571 antibody MERIT40 antibody NBA1 antibody BABAM1 antibody new component of the BRCA1-A complex antibody BRISC and BRCA1-A complex member 1 antibody new component of the BRCAA1 A complex antibody BRCA1-A complex subunit MERI |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) (4) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
HSPC142 antibody detects C19orf62 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human C19orf62-transfected 293T cells 10 % SDS-PAGE HSPC142 antibody dilution: 1:10000| Positive Control | human C19orf62-transfected 293T cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
