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Western blotting: use at a dilution of 1/500 - 1/1000. ELISA: use at a dilution of 1/5000 dilution. This gave an O.D.=1.0 in a 15 minute reaction using an HRP conjugated donkey anti rabbit (IgG) at 1/18000 and TMB as the substrate. Optimal dilutions / concentrations are best determined by the end user.:
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