Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HTRA2 antibody at 1:500 dilution.Application
HTRA2 antibody detects HTRA2 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg HepG2 whole cell lysate/extract 10 % SDS-PAGE HTRA2 antibody dilution: 1:1000Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HTRA2 antibody diluted at 1:1000.| Product Name | HTRA2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HTRA2 (HtrA serine peptidase 2)This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HTRA2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Rhesus Monkey, Chimpanzee, BovineMouse (80%), Rat (80%), Dog (81%), Rhesus Monkey (85%), Chimpanzee (85%), Bovine (81%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | OMI antibody PARK13 antibody PRSS25 antibody HTRA2 antibody HtrA-like serine protease antibody serine protease HTRA2 mitochondrial antibody serine proteinase OMI antibody serine protease 25 antibody protease serine 25 antibody Omi stress-regula |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) |
| Cellular Localization | Mitochondrion intermembrane space , Mitochondrion membrane; Single-pass membrane protein (Potential) |
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HTRA2 antibody at 1:500 dilution.
Application
HTRA2 antibody detects HTRA2 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg HepG2 whole cell lysate/extract 10 % SDS-PAGE HTRA2 antibody dilution: 1:1000
Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HTRA2 antibody diluted at 1:1000.| Positive Control | HeLa , HepG2 , Jurkat , Raji , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
