Application
Western blot analysis of extracts from MCF-7 cells, using HUCE1 Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | HUCE1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HUCE1Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HUCE1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HUCE1 |
|---|---|
| Target | HUCE1 |
| Molecular Weight(MW) | 50 kDa (note) |
| Tissue Specificity | HUCE1 Antibody detects endogenous levels of total HUCE1 protein. |
| Cellular Localization | Localizes at rDNA locus. |
Application
Western blot analysis of extracts from MCF-7 cells, using HUCE1 Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
