Application
Immunohistochemistry of human lymphoid tissue using anti-human IgA antibody RM128Application
Sandwich ELISA using RM128 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.Application
ELISA of human immunoglobulins shows RM128 reacted to both Human IgA1 & IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM128 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Application
A titer ELISA using RM128. The plate was coated with different amounts of human IgA. A serial dilution of RM128 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.| Product Name | Human IgA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM128] to Human IgA |
| Immunogen | Human IgA |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM128 |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIHC |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | immunoglobulin heavy constant alpha 1 IgA1 IGHA1 |
|---|---|
| Target | Human IgA |
| Tissue Specificity | This antibody reacts to human IgA, including both IgA1 and IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE. |
Application
Immunohistochemistry of human lymphoid tissue using anti-human IgA antibody RM128
Application
Sandwich ELISA using RM128 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Application
ELISA of human immunoglobulins shows RM128 reacted to both Human IgA1 & IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM128 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Application
A titer ELISA using RM128. The plate was coated with different amounts of human IgA. A serial dilution of RM128 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
