Application
Immunohistochemistry of human tonsil tissue using anti-human IgA2 antibody RM125Application
Sandwich ELISA using RM125 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.Application
ELISA of human immunoglobulins shows RM125 only reacted to Human IgA2. No cross reactivity with Human IgA1, IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM125 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Application
A titer ELISA using RM125. The plate was coated with different amounts of human IgA2. A serial dilution of RM125 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.| Product Name | Human IgA2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM125] to Human IgA2 |
| Immunogen | Human IgA2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM125 |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIHC |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | immunoglobulin heavy constant alpha 2 (A2m marker) IGHA2 |
|---|---|
| Target | Human IgA2 |
| Tissue Specificity | This antibody reacts to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE. |
Application
Immunohistochemistry of human tonsil tissue using anti-human IgA2 antibody RM125
Application
Sandwich ELISA using RM125 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
Application
ELISA of human immunoglobulins shows RM125 only reacted to Human IgA2. No cross reactivity with Human IgA1, IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM125 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Application
A titer ELISA using RM125. The plate was coated with different amounts of human IgA2. A serial dilution of RM125 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
