Application
Sandwich ELISA using RM119 as the capture antibody (100ng/well), and Biotinylated antihuman light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an HRP conjugated streptavidin.Application
ELISA of human immunoglobulins shows RM119 only reacted to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM119 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Application
A titer ELISA using RM119. The plate was coated with different amounts of human IgG3. A serial dilution of RM119 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.| Product Name | Human IgG3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM119] to Human IgG3 |
| Immunogen | Human IgG3 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM119 |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIHC |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IGHG3 immunoglobulin heavy constant gamma 3 (G3m marker) IgG3 |
|---|---|
| Target | Human IgG3 |
| Tissue Specificity | This antibody reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
Application
Sandwich ELISA using RM119 as the capture antibody (100ng/well), and Biotinylated antihuman light chains ( κ+ λ) antibody RM129 as the detection antibody, followed by an HRP conjugated streptavidin.
Application
ELISA of human immunoglobulins shows RM119 only reacted to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM119 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Application
A titer ELISA using RM119. The plate was coated with different amounts of human IgG3. A serial dilution of RM119 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
