Application
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Human IgG4 RabMAb (cat. #).Application
Western blot analysis on (A) 5 ng of IgG4, (B) 10 ng of IgG1, (C) 10 ng of IgG2, (D) 10 ng of IgG3, (E) human spleen, and (F) human plasma lysates using anti-human IgG4 RabMAb (cat. #).| Product Name | Human IgG4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP4420] to Human IgG4IgG is a glycoprotein that is produced by plasma cells in response to an immunogen. It is the only class of immunoglobulins that can cross the placenta, which is mediated by a receptor on placental cells in the Fc region (1). Human IgG4, one of 4 subclasses of IgG, contains a gamma 4 heavy chain and has a hinge region that is shorter than that of IgG1. No allotypes have been detected on the heavy chains of IgG4. Its two primary effector functions are activating complements and binding to the FcgR of effector cells to initiate phagocytosis (2). |
| Immunogen | A synthetic peptide corresponding to residues in the hinge region of Human IgG4 was used as an immunogen. It does not cross-react with IgG1, IgG2, or IgG3. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP4420 |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB: Use at 1:1,000-10,000 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Target | Human IgG4 |
|---|
Application
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Human IgG4 RabMAb (cat. #).
Application
Western blot analysis on (A) 5 ng of IgG4, (B) 10 ng of IgG1, (C) 10 ng of IgG2, (D) 10 ng of IgG3, (E) human spleen, and (F) human plasma lysates using anti-human IgG4 RabMAb (cat. #).| Application Notes | For WB: Use at 1:1,000-10,000 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
