Application
Sandwich ELISA using RM120 as the capture antibody, and Biotinylated anti-human light chains( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.Application
Western Blot of human, mouse, rat, and goat IgG shows RM120 reacted to human IgG4 both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced). No cross reactivity with other isotype of human IgG, and mouse, rat, or goat IgG.Application
A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Application
Immunohistochemistry of human lymphoidtissue using anti-human IgG4 antibody RM120.| Product Name | Human IgG4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM120] to Human IgG4 |
| Immunogen | Peptide corresponding to the hinge region of Human IgG4 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM120 |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFIHCWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | immunoglobulin heavy constant gamma 4 (G4m marker) IGHG4 |
|---|---|
| Target | Human IgG4 |
| Tissue Specificity | This antibody reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
Application
Sandwich ELISA using RM120 as the capture antibody, and Biotinylated anti-human light chains( κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin.
Application
Western Blot of human, mouse, rat, and goat IgG shows RM120 reacted to human IgG4 both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced). No cross reactivity with other isotype of human IgG, and mouse, rat, or goat IgG.
Application
A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Application
Immunohistochemistry of human lymphoidtissue using anti-human IgG4 antibody RM120.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
