Application
Immunohistochemistry of human tonsil using anti-lambda light chain antibody RM127
Application
ELISA of human immunoglobulins shows RM127 reacted to the lambda light chain of human immunoglobulins. No cross reactivity with the kappa light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Application
A titer ELISA using RM127. The plate was coated with different amounts of human IgG3λ. A serial dilution of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.