Application
Immunohistochemical analysis of paraffin - embedded human pancreas carcinoma showing cytoplasmic and membrane location using 4E - BP1 antibody with DAB staining.Application
Western blot analysis using 4E - BP1 monoclonal antibody against truncated 4E - BP1 recombinant protein and A431 cell lysate.| Product Name | 4E-BP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | 4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13 kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24 kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.1) Pause, A. et al. 1994.Nature. 371:762–767. |
| Immunogen | Ni-NTA purified truncated recombinant 4E-BP1 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:500 - 1:1,000 IHC(P):1:500 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | EIF4EBP1 |
|---|
Application
Immunohistochemical analysis of paraffin - embedded human pancreas carcinoma showing cytoplasmic and membrane location using 4E - BP1 antibody with DAB staining.
Application
Western blot analysis using 4E - BP1 monoclonal antibody against truncated 4E - BP1 recombinant protein and A431 cell lysate.| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:1,000 IHC(P):1:500 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | 4E-BP1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
