Application
Total protein extract of normal human cerebral cortex separated as a strip on a 3-12.5% gradient SDS-PAGE gel and Western blotted. The blot was probed with NB 600-755.| Product Name | Huntingtin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to HuntingtinThe combined use of clones ab7666, ab7667 and ab7668 demonstrate that huntingtin is enriched in neuronal cells in the brain. |
| Immunogen | Recombinant fragment, corresponding to amino acids 997-1276 of Huntingtin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | HDA3E10 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FIPWB |
| We recommend the following starting dilutions: immunofluorescence, Immunohistochemistry-Frozen, immunoprecipitation, Western Blot. Optimal concentration should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | HUNTINGTON CHOREA IT15 IT 15 15194 HTT 29424 3064 HDH Huntington's disease protein homolog Huntington disease protein HD protein ZHD |
|---|---|
| Target | Huntingtin |
| Tissue Specificity | This reacts with an epitope corresponding to the HDA region (amino acids 1171 - 1177) of the huntingtin protein. |
| Cellular Localization | Cytoplasmic |
Application
Total protein extract of normal human cerebral cortex separated as a strip on a 3-12.5% gradient SDS-PAGE gel and Western blotted. The blot was probed with NB 600-755.| Application Notes | We recommend the following starting dilutions: immunofluorescence, Immunohistochemistry-Frozen, immunoprecipitation, Western Blot. Optimal concentration should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4ºC for short-term storage. Aliquot and store at -20°C for long-term. Avoid repeated freezing and thawing. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
