Application
Western blot analysis of extracts of various cell lines, using HUS1 antibody.Application
Immunohistochemistry of paraffin-embedded human kidney using HUS1 antibody at dilution of 1:200 (x400 lens).Application
Immunohistochemistry of paraffin-embedded human stomach cancer using HUS1 antibody at dilution of 1:200 (x400 lens).Application
Immunofluorescence analysis of U20S cell using HUS1 antibody. Blue: DAPI for nuclear staining.Application
Immunofluorescence analysis of U2OS cell using HUS1 antibody. Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.| Product Name | HUS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HUS1The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011] |
| Immunogen | Recombinant protein of human HUS1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HUS1 checkpoint clamp component hHUS1 |
|---|---|
| Target | HUS1 |
| Molecular Weight(MW) | 32 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using HUS1 antibody.
Application
Immunohistochemistry of paraffin-embedded human kidney using HUS1 antibody at dilution of 1:200 (x400 lens).
Application
Immunohistochemistry of paraffin-embedded human stomach cancer using HUS1 antibody at dilution of 1:200 (x400 lens).
Application
Immunofluorescence analysis of U20S cell using HUS1 antibody. Blue: DAPI for nuclear staining.
Application
Immunofluorescence analysis of U2OS cell using HUS1 antibody. Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
