Application
Western blot experiment using HUWE1 Antibody at 1:90000 dilution to detect 100ng of 26kDa recombinant HUWE1 protein antigen.| Product Name | HUWE1 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HUWE1 (HECT, UBA and WWE domain containing 1)This gene encodes a member of the HECT E3 ubiquitin ligase family. The HECT domain lies in the C-terminus and contains the active-site cysteine which forms an intermediate ubiquitin-thioester bond. E3 family members are divided into three subfamilies based on their protein-protein interaction domains; this gene encodes a member of the SI(ngle)-HECT E3 subfamily. In lung, breast, and colorectal carcinomas, this gene is highly expressed. Mutations in this gene has been found in 3 unrelated families with X-linked syndromic mental retardation, Turner type. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human HUWE1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Predicted Reactivity | Human, Mouse, Rhesus Monkey, BovineHuman (100%), Mouse (96%), Rhesus Monkey (99%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ARF-BP1 antibody HECTH9 antibody HSPC272 antibody Ib772 antibody KIAA0312 antibody LASU1 antibody MULE antibody UREB1 antibody HUWE1 antibody upstream regulatory element-binding protein 1 antibody large structure of UREB1 antibody E3 ubiquitin-p |
|---|---|
| Molecular Weight(MW) | 482 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Western blot experiment using HUWE1 Antibody at 1:90000 dilution to detect 100ng of 26kDa recombinant HUWE1 protein antigen.| Positive Control | Target recombinant protein |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
