Application
IARS antibody detects IARS protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IARS antibody diluted at 1:4000.Application
Immunoprecipitation of IARS protein from 293T whole cell extracts using 5 μg of IARS antibody . Western blot analysis was performed using IARS antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
IARS antibody detects IARS protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. IARS antibody diluted at 1:500.| Product Name | IARS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IARS (isoleucyl-tRNA synthetase)Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IARS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevis, BovineMouse (92%), Xenopus laevis (80%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PRO0785 IRS IARS1 ILERS isoleucyl-tRNA synthetase ILRS |
|---|---|
| Target | IARS |
| Molecular Weight(MW) | 144 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
IARS antibody detects IARS protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IARS antibody diluted at 1:4000.
Application
Immunoprecipitation of IARS protein from 293T whole cell extracts using 5 μg of IARS antibody . Western blot analysis was performed using IARS antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
IARS antibody detects IARS protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. IARS antibody diluted at 1:500.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
