Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE ICA69 antibody diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded A2780-CP70 xenograft, using ICA1 antibody at 1:100 dilution.| Product Name | ICA1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ICA1 (islet cell autoantigen 1, 69kDa)This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ICA1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Rhesus Monkey, BovineMouse (99%), Rat (100%), Zebrafish (83%), Xenopus laevis (86%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ICA69 antibody ICAp69 antibody ICA1 antibody p69 antibody islet cell autoantigen p69 antibody 69 kDa islet cell autoantigen antibody diabetes mellitus type I autoantigen antibody islet cell autoantigen 1 isoform antibody islet cell autoantigen 1 an |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (5) |
| Cellular Localization | Cytoplasm , cytosol , Golgi apparatus membrane; Peripheral membrane protein (Potential) , Cytoplasmic vesicle , secretory vesicle membrane; Peripheral membrane protein (Potential) , secretory vesicle , synaptic vesicle membrane; Peripheral membrane protei |
Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE ICA69 antibody diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded A2780-CP70 xenograft, using ICA1 antibody at 1:100 dilution.| Positive Control | A549 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
