Application
Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ICAD (cleaved Asp224) antibody diluted at 1:1000.| Product Name | ICAD (cleaved Asp224) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ICAD (cleaved Asp224) (DNA fragmentation factor, 45kDa, alpha polypeptide)Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ICAD (cleaved Asp224). The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DFF-45 DFF1 DFFA DNA fragmentation factor 45kDa alpha polypeptide ICAD |
|---|---|
| Target | ICAD (cleaved Asp224) |
| Molecular Weight(MW) | 37 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ICAD (cleaved Asp224) antibody diluted at 1:1000.| Positive Control | Jurkat , Jurkat (10uM etoposide treatment for 24h) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
