Application
Western blot analysis of extracts of various cell lines, using DFFA antibody.Application
Immunohistochemistry of paraffin-embedded human stomach using DFFA antibody at dilution of 1:100 (x400 lens).Application
Immunofluorescence analysis of HeLa cell using DFFA antibody. Blue: DAPI for nuclear staining.| Product Name | ICAD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ICADApoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human DFFA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DFF1 DFFA DNA fragmentation factor subunit alpha DFF45 |
|---|---|
| Target | ICAD |
Application
Western blot analysis of extracts of various cell lines, using DFFA antibody.
Application
Immunohistochemistry of paraffin-embedded human stomach using DFFA antibody at dilution of 1:100 (x400 lens).
Application
Immunofluorescence analysis of HeLa cell using DFFA antibody. Blue: DAPI for nuclear staining.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
