Application
Western Blot analysis of Hela cell lysate probed with Rabbit anti Human DFF used at 1 (A) and 2 (B) ug/mlApplication
Western blot analysis of DFF45 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) cell lysates with DFF45 antibody at 2μg/ml.| Product Name | ICAD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ICADApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the Caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by Caspase 3 during apoptosis. A mouse homologue of human DFF was identified as inhibitor of caspase activated deoxyribonuclease (ICAD). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death. |
| Immunogen | Peptide: amino acids 2-21 at the N-terminus of human ICAD |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IPWB |
| For IP: Use at a dilution of 1:1000-1:2,000. For WB: Use at a dilution of 1:1,000-1:2,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Anti-DFF 1 Anti-DFF 45 Anti-DFF alpha Anti-DFF1 Anti-DFF35 Anti-DFF45 Anti-DFFA Anti-DNA Fragmentation Factor Alpha Subunit Anti-ICAD Anti-Inhibitor of Caspase Activated DNase DFF 1 antibody DFF 45 antibody DFF alpha antibody DFF1 antibody DFF35 antibody D |
|---|---|
| Target | DFF |
| Tissue Specificity | Recognizes the non-cleaved and cleaved DFF |
Application
Western Blot analysis of Hela cell lysate probed with Rabbit anti Human DFF used at 1 (A) and 2 (B) ug/ml
Application
Western blot analysis of DFF45 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) cell lysates with DFF45 antibody at 2μg/ml.| Application Notes | For IP: Use at a dilution of 1:1000-1:2,000. For WB: Use at a dilution of 1:1,000-1:2,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline, pH 7.4, containing 0.02% sodium azide |
