Application
Western blot analysis of extracts from HUVEC cells using ICAM-1 (Phospho-Tyr512) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).| Product Name | ICAM1 / CD54 (phospho Tyr512) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ICAM-1 (Phospho Tyr512)ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 512 (K-K-Y(p)-R-L) derived from Human ICAM-1. |
| Modification | p-Tyr512 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BB2 CD54 P3.58 ICAM1 intercellular adhesion molecule 1 intercellular adhesion molecule 1 (CD54) human rhinovirus receptor major group rhinovirus receptor cell surface glycoprotein P3.58 human rhinovirus receptor ICAM-1 |
|---|---|
| Target | ICAM-1 (Phospho Tyr512) |
| Molecular Weight(MW) | 92 (note) |
Application
Western blot analysis of extracts from HUVEC cells using ICAM-1 (Phospho-Tyr512) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
