WB
Western blot analysis of ICAM1 using anti-ICAM1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.WB
Western blot analysis of ICAM1 using anti-ICAM1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ICAM1 using anti-ICAM1 antibody. ICAM1 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. The tissue section was incubated with rabbit anti-ICAM1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
IF analysis of ICAM1 using anti-ICAM1 antibody. ICAM1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ICAM1 Antibody at a dilution of 1:100.Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of PC-3 cells using anti-ICAM1 antibody. Overlay histogram showing PC-3 cells stained with A00171. To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ICAM1 Antibody at 1:100 dilution for 30 min at 20°C. Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ICAM1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human ICAM1 recombinant protein (Position: Q28-R268). Human ICAM1 shares 55.5% and 55.8% amino acid (aa) sequence identity with mouse and rat ICAM1, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ICAM1 |
|---|---|
| Gene Synonyms | BB2 CD54 P3.58 |
| Gene Full Name | intercellular adhesion molecule 1 |
| Gene Summary | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008] |
| Alternative Names | BB2 CD54 ICAM 1 ICAM1 ICAM-1 P3.58 Ly 47 MALA 2 |
| Molecular Weight(MW) | 59kDa(Observed MW 90-110kDa) |
| Cellular Localization | Membrane. |
WB
Western blot analysis of ICAM1 using anti-ICAM1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
WB
Western blot analysis of ICAM1 using anti-ICAM1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse HEPA1-6 whole cell lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ICAM1 using anti-ICAM1 antibody. ICAM1 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. The tissue section was incubated with rabbit anti-ICAM1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
IF analysis of ICAM1 using anti-ICAM1 antibody. ICAM1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-ICAM1 Antibody at a dilution of 1:100.Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of PC-3 cells using anti-ICAM1 antibody. Overlay histogram showing PC-3 cells stained with A00171. To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ICAM1 Antibody at 1:100 dilution for 30 min at 20°C. Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
