WB
Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody at 1/5,000 dilution. Lane 1: HepG2 cell lysate, Lane 2: K-562 cell lysate (low expression), Lane 3: Raji cell lysate, Lane 4: Ramos cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ICAM1 antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ICAM1 antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-ICAM1 antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Species: Human, Site: colon cancer, Sample: Paraffin-embedded section, Antibody concentration: 1/1,000.| Product Name | ICAM1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | ecombinant protein within human ICAM1 aa 1-480. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:5000 IHC:1:8000 IF-P:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ICAM1 |
|---|---|
| Gene Synonyms | BB2 CD54 P3.58 |
| Gene Full Name | intercellular adhesion molecule 1 |
| Gene Summary | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008] |
| Alternative Names | Antigen identified by monoclonal antibody BB2 antibody BB 2 antibody BB2 antibody CD 54 antibody CD_antigen=CD54 antibody CD54 antibody Cell surface glycoprotein P3.58 antibody Human rhinovirus receptor antibody ICAM 1 antibody ICAM-1 antibody Antigen identified by monoclonal antibody BB2 antibody BB 2 antibody BB2 antibody CD 54 antibody CD_antigen=CD54 antibody CD54 antibody Cell surface glycoprotein P3.58 antibody Human rhinovirus receptor antibody ICAM 1 antibody ICAM-1 antibody ICAM1 antibody ICAM1_HUMAN antibody intercellular adhesion molecule 1 (CD54) antibody intercellular adhesion molecule 1 (CD54) human rhinovirus receptor antibody Intercellular adhesion molecule 1 antibody Major group rhinovirus receptor antibody MALA 2 antibody MALA2 antibody MyD 10 antibody MyD10 antibody P3.58 antibody Surface antigen of activated B cells antibody Surface antigen of activated B cells BB2 antibody |
| Molecular Weight(MW) | 58kDa(Observed band size: 110kDa) |
| Cellular Localization | Membrane. |
WB
Western blot analysis of ICAM1 on different lysates with Rabbit anti-ICAM1 antibody at 1/5,000 dilution. Lane 1: HepG2 cell lysate, Lane 2: K-562 cell lysate (low expression), Lane 3: Raji cell lysate, Lane 4: Ramos cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ICAM1 antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ICAM1 antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-ICAM1 antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Species: Human, Site: colon cancer, Sample: Paraffin-embedded section, Antibody concentration: 1/1,000.| Application Notes | WB:1:5000 IHC:1:8000 IF-P:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
