Application
Sample (30 ug of whole cell lysate) A: K562 7.5% SDS PAGE diluted at 1:3000Application
ICAM2 antibody [N1C3] detects ICAM2 protein at cell membrane by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ICAM2 protein stained by ICAM2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | ICAM2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ICAM2 (intercellular adhesion molecule 2)The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ICAM2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (85%), Chimpanzee (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD102 antibody, ICAM2 antibody, ICAM-2 antibody, intercellular adhesion molecule 2 antibody |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) (6) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Sample (30 ug of whole cell lysate) A: K562 7.5% SDS PAGE diluted at 1:3000
Application
ICAM2 antibody [N1C3] detects ICAM2 protein at cell membrane by immunofluorescent analysis. Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ICAM2 protein stained by ICAM2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | K562 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
