| Product Name | ICEBERG antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ICEBERGA novel protein, ICEBERG, an inhibitor of pro-caspase 1activation, has been studied. It consists essentially only of a CARD, inhibits generation of IL-1b by interacting with caspase-1 and preventing association with RIP 2. The ICEBERG functions as a competitive antagonist of RIP 2/pro-caspase 1 interactions and inhibits caspase 1 dependent pro-L-1b secretion in mononuclear cells. ICEBERG is induced by proinflammatory stimuli, suggesting that it may be part of a negative feedback loop. |
| Immunogen | Synthetic peptide (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,IP,WB |
| Immunoprecipitation: use at a concentration of 3.0 - 5.0 μg / extract from 10^7 cells. Western Blotting: use at a concentration of 0.5 - 2.0 μg/ml. ELISA: use at a concentration of 0.1 - 1.0 μg/ml. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of approximately 10 kDa rat brain lysate, which corresponds to the predicted molecular weight of ICEBERG. Samples were boiled prior to subjecting to a15% SDS-polyacrylamide gel electrophoresis. | |
| Species Reactivity | Human,Rat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ICEBERG, UNQ5804, pseudo-ICE, pseudo ICE, pseudoICE |
|---|---|
| Target | ICEBERG |
| Tissue Specificity | This antibody reacts predominantly with ICEBERG. |
| Application Notes | Immunoprecipitation: use at a concentration of 3.0 - 5.0 μg / extract from 10^7 cells. Western Blotting: use at a concentration of 0.5 - 2.0 μg/ml. ELISA: use at a concentration of 0.1 - 1.0 μg/ml. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of approximately 10 kDa rat brain lysate, which corresponds to the predicted molecular weight of ICEBERG. Samples were boiled prior to subjecting to a15% SDS-polyacrylamide gel electrophoresis. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide |
