Application
Lane 1,2: AmSO4 fractionated Lane 3,4: HCIC Purified Lane 5, 6: Protein A PurifiedApplication
Sec HPLC ProfileApplication
Zf anti‐Id1 is specific. (Left) CBB‐stained SDS PAGE. (Right) Duplicate western blot Lane 1: Standards Lane 2: 1ml Id1 in‐vitro TNTApplication
Zf anti‐Id1 WB shows specificity amongst all other Zf embryo proteins Lane 1: Id1 in‐vitro TNT Lane 2: Standards Lane 3: De‐yolked 24 hpf D. rerio embryo extract (band shift vs. lane 1 due to lack of glycosylation of TNT sample)Application
Zf anti-Id1 WB shows differentiation between Id proteins. Lane 1: loading control Lane 2: Id1 in‐vitro TNT Lane 3: Id3 in‐vitro TNT| Product Name | Id1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Id1The Id proteins (inhibitors of DNA binding /differentiation) represent a family of dominant negative regulators of the basic helix-loop-helix transcription factors whose activities result in delayed cell differentiation and prolonged proliferation. |
| Immunogen | Synthetic peptide derived from the C-terminal region of zebrafish Id1 (Genbank accession# NP_571320.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a suggested starting dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Zebra Fish |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Target | Id1 |
|---|
Application
Lane 1,2: AmSO4 fractionated Lane 3,4: HCIC Purified Lane 5, 6: Protein A Purified
Application
Sec HPLC Profile
Application
Zf anti‐Id1 is specific. (Left) CBB‐stained SDS PAGE. (Right) Duplicate western blot Lane 1: Standards Lane 2: 1ml Id1 in‐vitro TNT
Application
Zf anti‐Id1 WB shows specificity amongst all other Zf embryo proteins Lane 1: Id1 in‐vitro TNT Lane 2: Standards Lane 3: De‐yolked 24 hpf D. rerio embryo extract (band shift vs. lane 1 due to lack of glycosylation of TNT sample)
Application
Zf anti-Id1 WB shows differentiation between Id proteins. Lane 1: loading control Lane 2: Id1 in‐vitro TNT Lane 3: Id3 in‐vitro TNT| Application Notes | For WB: Use at a suggested starting dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris buffer, 140 mM NaCl, pH 7.4 containing 0.01% sodium azide |
