Application
FACS analysis of U251 cells (right histogram) compared to a negative control cell (left histogram) using ID1 antibody.Application
Confocal IF analysis of U-251MG cells using ID1 antibody (green). DAPI was used to stain the cell nuclear (blue).Application
WB analysis of U251 cell lysate (35ug) using ID1 antibody.Application
WB analysis of mouse heart tissue lysate (35ug) using ID1 antibody.Application
IHC-P analysis of human pancreas tissue using ID1 antibody.| Product Name | ID1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ID1The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-93 amino acids from the central region of human ID1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACS,ICC/IF,IHC-P,WB |
| WB : 1:1000,IHC |
|
| Species Reactivity | Human,Mouse |
| Predicted Reactivity | 251 |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | ID , inhibitor of DNA binding 1, dominant negative helix-loop-helix protein , bHLHb24 |
|---|---|
| Target | ID1 |
Application
FACS analysis of U251 cells (right histogram) compared to a negative control cell (left histogram) using ID1 antibody.
Application
Confocal IF analysis of U-251MG cells using ID1 antibody (green). DAPI was used to stain the cell nuclear (blue).
Application
WB analysis of U251 cell lysate (35ug) using ID1 antibody.
Application
WB analysis of mouse heart tissue lysate (35ug) using ID1 antibody.
Application
IHC-P analysis of human pancreas tissue using ID1 antibody.| Application Notes | WB : 1:1000,IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide |
