Application
IHC with clone 195-14. Invasive ductal carcinoma human triple neg. mammary tumor (negative for PR, negative for ER, and negative for Her-2/Neu) with several tumor cells (TC) expressing Id1. (Perk et. al.)Application
Western Blot with clone 195-14. Lane 1: Whole cell extract from Id1- /- mouse embryonic fibroblasts. Lane 2: Whole cell extract from Id1+/+ mouse embryonic fibroblasts. Lane 3: Whole cell extract from HeLa cells. (Perk et.al.)| Product Name | Id1 antibody [195-14] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [195-14] to Id1There are four members of the Id protein family, Id1, Id2, Id3, and Id4. These proteins were initially discovered as proteins involved in the negative control of cell differentiation. Id proteins act as a negative regulator of transcription through physical interaction with a group of transcription factors known as bHLH (basic helix-loop-helix) proteins. Id proteins interact with bHLH proteins in a manner that prevents DNA binding to the HLH proteins. Because of this activity, the group of proteins were named as Id (for inhibitor of DNAbinding). Id proteins have also been found to bind with a number of other proteins such as Rb, Ets, Paz, MIDA-1 and SREBP-1c. Id proteins may play a central role in coordinating gene expression, cell proliferation, tumorigenesis, and angiogenesis. Id proteins have been found to be over-expressed in many types, including Glioblastoma, Medulloblastoma, Neuroblastoma, Pancreatic Cancer, Thyroid Cancer, Squamous Cell Carcinoma, Breast Carcinoma, Endometrial Cancer, Cervical Cancer, Melanoma, and Retinoblastoma. There is a growing body of evidence that Id1 and Id3 play a central role in angiogenesis. Experiments in Id1-/-, Id3-/- knockout mice indicated that with the loss of Id expression there was no vascularization and no subsequent growth of tumors. |
| Immunogen | Recombinant full length mouse Id1 recombinant protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 195-14 |
| Host Species | Rabbit |
| Tested Applications | IHC,IHC-P,WB |
| IHC-P: Use at 1/100 dilution and incubate for 2 hours at room temperature | |
| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | Id1, inhibitor of DNA binding1, dominant negative helixloophelix protein, dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein), inhibitor of differentiation1, inhibitor of differentiation 1, dJ857M17.1.2, Class B basic heli |
|---|---|
| Target | Id1 |
Application
IHC with clone 195-14. Invasive ductal carcinoma human triple neg. mammary tumor (negative for PR, negative for ER, and negative for Her-2/Neu) with several tumor cells (TC) expressing Id1. (Perk et. al.)
Application
Western Blot with clone 195-14. Lane 1: Whole cell extract from Id1- /- mouse embryonic fibroblasts. Lane 2: Whole cell extract from Id1+/+ mouse embryonic fibroblasts. Lane 3: Whole cell extract from HeLa cells. (Perk et.al.)| Application Notes | IHC-P: Use at 1/100 dilution and incubate for 2 hours at room temperature |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4 °C. DO NOT FREEZE. Product may precipitate upon freezing. |
| Storage Buffer | Cell culture supernatant with 0.25% BSA and 0.05% sodium azide |
