Application
Sample (30 ug of whole cell lysate) A: HCT116 15% SDS PAGE diluted at 1:1000Application
ID2 antibody [N1C3] detects ID2 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ID2 antibody [N1C3] diluted at 1:500.| Product Name | ID2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ID2 (inhibitor of DNA binding 2, dominant negative helix-loop-helix protein)The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene. [provided by RefSeq] |
| Immunogen | Full length human ID2 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevis, Pig, Chicken, BovineMouse (98%), Xenopus laevis (82%), Pig (98%), Chicken (94%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GIG8 antibody ID2A antibody ID2H antibody MGC26389 antibody bHLHb26 antibody ID2 antibody class B basic helix-loop-helix protein 26 antibody inhibitor of differentiation 2 antibody DNA-binding protein inhibitor ID2 antibody DNA-binding protein inh |
|---|---|
| Target | ID2 |
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: HCT116 15% SDS PAGE diluted at 1:1000
Application
ID2 antibody [N1C3] detects ID2 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ID2 antibody [N1C3] diluted at 1:500.| Positive Control | HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
