Application
Flow cytometric analysis of Hela cells, using anti-ID3 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Flow cytometric analysis of Jurkat cells, using anti-ID3 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Immunohistochemical staining of paraffin-embedded liver tissue using anti-ID3mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID3.| Product Name | ID3 antibody [10D3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [10D3] to ID3ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors. |
| Immunogen | Full length human recombinant protein of human ID3 (NP_002158) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10D3 |
| Host Species | Mouse |
| Tested Applications | FACS,IHC,IHC-P,WB |
| ICC/IF 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HEIR 1, HEIR1 |
|---|---|
| Target | ID3 |
| Tissue Specificity | is specific for human ID3 (NP_002158). |
Application
Flow cytometric analysis of Hela cells, using anti-ID3 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Flow cytometric analysis of Jurkat cells, using anti-ID3 antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Immunohistochemical staining of paraffin-embedded liver tissue using anti-ID3mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID3.| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
