Application
Western blot analysis of extracts of various tissues, using IDE antibody.Application
Immunohistochemistry of paraffin-embedded human kidney using IDE antibody at dilution of 1:100 (x400 lens).Application
Immunofluorescence analysis of A549 cell using IDE antibody. Blue: DAPI for nuclear staining.| Product Name | IDE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IDEThis gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009] |
| Immunogen | Recombinant protein of human IDE |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IHC,IHC-P,WB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:10 - 1:100 | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | INSULYSIN , insulin-degrading enzyme |
|---|---|
| Target | IDE |
| Molecular Weight(MW) | 118 kDa (note) |
Application
Western blot analysis of extracts of various tissues, using IDE antibody.
Application
Immunohistochemistry of paraffin-embedded human kidney using IDE antibody at dilution of 1:100 (x400 lens).
Application
Immunofluorescence analysis of A549 cell using IDE antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:10 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
