Application
Western blot analysis of extracts from HuvEc cells, using IF3EI Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | IF3EI antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to IF3EIComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IF3EI. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISA,WB |
| ELISA: 1:1000 Optimal dilutions should be determined experimentally by the end user. |
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| Species Reactivity | Human,Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Eukaryotic translation initiation factor3 subunit6 interacting protein, Eukaryotic translation initiation factor 3 subunit 6 interacting protein, IF3EI, EIF3S6IP, HSPC021/HSPC025, IF3I |
|---|---|
| Target | IF3EI |
| Molecular Weight(MW) | 66 kDa (note) |
| Tissue Specificity | IF3EI Antibody detects endogenous levels of total IF3EI protein. |
Application
Western blot analysis of extracts from HuvEc cells, using IF3EI Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:1000 Optimal dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
