Application
IHC analysis of colchicine injected rat brain (including the cortex and median eminence) tissue using IFN gamma antibody at a concentration of 0.25 mg/ml. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent.| Product Name | IFN gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IFN gammaan immune molecule produced by T lymphocytes in response to mitogens or antigens [RGD, Feb 2006] |
| Immunogen | Recombinant Rat IFN-γ |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 µg/ml. For ELISA: Use at a concentration of 0.5-2.0 μg/ml. For IHC: Use at a concentration of 0.25 ug/ml. Optimal dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Gene Synonyms | IFNG2, IFNG2, Ifng, interferon gammaAntibody |
|---|
Application
IHC analysis of colchicine injected rat brain (including the cortex and median eminence) tissue using IFN gamma antibody at a concentration of 0.25 mg/ml. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent.| Application Notes | We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 µg/ml. For ELISA: Use at a concentration of 0.5-2.0 μg/ml. For IHC: Use at a concentration of 0.25 ug/ml. Optimal dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.2 |
