Application
IFT20 antibody detects IFT20 protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IFT20 protein stained by IFT20 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | IFT20 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IFT20 (intraflagellar transport 20 homolog (Chlamydomonas))Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IFT20. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | intraflagellar transport 20 homolog (Chlamydomonas) |
|---|---|
| Target | IFT20 |
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Golgi apparatus , cis-Golgi network (By similarity) , Centrosome , centriole (By similarity) , Cell projection , cilium basal body (By similarity) |
Application
IFT20 antibody detects IFT20 protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IFT20 protein stained by IFT20 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
