Application
IFT80 antibody detects IFT80 protein at centrosome by immunofluorescent analysis. Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min. Green: IFT80 protein stained by IFT80 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using IFT80 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE diluted at 1:500| Product Name | IFT80 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to IFT80 (intraflagellar transport 80 homolog (Chlamydomonas))The IFT80 gene encodes a protein with 7 WD40 domains that is a component of the intraflagellar transport (IFT) complex B (Beales et al., 2007 [PubMed 17468754]). The IFT is essential for the development and maintenance of motile and sensory cilia.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human IFT80. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Chimpanzee, BovineMouse (97%), Rat (97%), Zebrafish (81%), Chimpanzee (100%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATD2 antibody, KIAA1374 antibody, MGC126543 antibody, WDR56 antibody, IFT80 antibody, WD repeat-containing protein 56 antibody, intraflagellar transport protein 80 homolog antibody, WD repeat domain 56 antibody, intraflagellar transport 80 homolog (Chlamyd |
|---|---|
| Molecular Weight(MW) | 88 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
IFT80 antibody detects IFT80 protein at centrosome by immunofluorescent analysis. Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min. Green: IFT80 protein stained by IFT80 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using IFT80 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE diluted at 1:500| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
